Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells
By V. Planat-Bénard, Claudine Ménard, Mireille André, M. Pucéat, A. Perez, Jose Manuel García-Verdugo, L. Pénicaud, L. Casteilla. Circulation Research, 2004
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Abstract— Cardiomyocyte regeneration is limited in adult life. Thus, the identification of a putative source of cardiomyocyte progenitors is of great interest to provide a usable model in vitro and new perspective in regenerative therapy. As adipose tissues were recently demonstrated to contain pluripotent stem cells, the emergence of cardiomyocyte phenotype from adipose-derived cells was investigated. We demonstrated that rare beating cells with cardiomyocyte features could be identified after culture of adipose stroma cells without addition of 5-azacytidine. The cardiomyocyte phenotype was first identified by morphological observation, confirmed with expression of specific cardiac markers, immunocytochemistry staining, and ultrastructural analysis, revealing the presence of ventricle- and atrial-like cells. Electrophysiological studies performed on early culture revealed a pacemaker activity of the cells. Finally, functional studies showed that adrenergic agonist stimulated the beating rate whereas cholinergic agonist decreased it. Taken together, this study demonstrated that functional cardiomyocyte-like cells could be directly obtained from adipose tissue. According to the large amount of this tissue in adult mammal, it could represent a useful source of cardiomyocyte progenitors.
Subfields: Molecular Biology, Surgery
View Paper: https://www.semanticscholar.org/paper/a0712ef353a3622cdca0eaaccbac638b59a800b0
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Citations in the corpus
- Cell-Assisted Lipotransfer: Supportive Use of Human Adipose-Derived Cells for Soft Tissue Augmentation with Lipoinjection (2006, positive): “Human ASCs were shown by several in vitro and in vivo studies to differentiate into adipogenic, osteogenic, 3–7 chondrogenic, 8–10 myogenic, 11,12 cardiomyogenic, 13,14 and neurogenic 15–17 lineages.”
- Unchain my heart: the scientific foundations of cardiac repair. (2005, background): “org Volume 115 Number 3 March 2005 On the horizon of being tested for potential clinical application are other progenitor/stem cell populations: fat tissue–derived multipotent stem cells (36); multipotential cells from bone marrow or skeletal muscle (minuscule subpopulations, distinct from the unfra”
- Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates (2006, background): “Other studies showed that CD34þCD31 cells differentiated into endothelial cells and contributed to neovascularization (Miranville et al., 2004; Journal of Cellular Physiology DOI 10.1002/jcp Planat-Benard et al., 2004b).”