Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.

By Bernd Zetsche, Jonathan Gootenberg, Omar Abudayyeh, I. Slaymaker, Kira S. Makarova, Patrick Essletzbichler, Sara Volz, J. Keith Joung, J. van der Oost, Aviv Regev and 2 more. 2015

4,241 citations recorded by the source corpus.

Subfields: Business and International Management, Cardiology and Cardiovascular Medicine, Molecular Biology

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Citations in the corpus

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity (2018, neutral): “Primers and templates used in the synthesis of all sgRNA plasmids used in this work are listed in Supplementary Tables 8, 9, 11, 12, 15, and 16.”
CRISPR/Cas9 in Genome Editing and Beyond. (2016, background): “When targeted to paired adjacent sequences, the FokI domains of these programmable, site-specific nucleases form a dimer that activates the nuclease activity, thus creating a DSB near their binding sites (Figure 1a).”
CRISPRDetect: A flexible algorithm to define CRISPR arrays (2016, background): “In Type I, Type II and Type V systems, the bases at one end of the spacer are usually part of a ‘ seed ’ sequence, that is critical for base-pairing, target recognition and interference [36 – 40].”
CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation (2019, positive): “Indeed, we assume these trials extend to other INDEL-producing gene editing systems that have been applied in human cells such as the CRISPR endonuclease Cpf1 28 .”
RNA Editing with CRISPR-Cas13 (2017, methodology): “Current editing tools, based on programmable nucleases such as the prokaryotic CRISPR-associated nucleases Cas9 (1–4) or Cpf1 (5), have been widely adopted for mediating targeted DNA cleavage, which in turn drives targeted gene disruption through nonhomologous end joining (NHEJ) or precise gene edit”
The Biology of CRISPR-Cas: Backward and Forward. (2018, positive): “…and suf-ﬁcient base pairing between the crRNA and target DNA, Cas12a and Cas12b cleave both DNA strands, resulting in staggered double-stranded breaks with 5-and 7-nt overhangs distal to the PAM, respectively (Fonfara et al., 2016; Shmakov et al., 2015; Yang et al., 2016; Zetsche et al., 2015).”