Image-based analysis of lipid nanoparticle–mediated siRNA delivery, intracellular trafficking and endosomal escape

By J. Gilleron, W. Querbes, Anja Zeigerer, A. Borodovsky, Giovanni Marsico, Undine Schubert, K. Manygoats, S. Seifert, Cordula Andree, M. Stöter and 10 more. Nature Biotechnology, 2013

1,456 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9ff23fddbc3af9e218dc679dcf57bdbf7b5f84ed

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Lipid Nanoparticle Systems for Enabling Gene Therapies. (2017, background): “The potential for improvement is clear in that <5% of endocytosed LNP siRNA is actually released into the cell cytosol.(40) Up to 60% is recycled to the extracellular medium,(41) and much of the remaining endocytosed LNP siRNA proceeds to other non-productive endpoints such as degradation in lysosom”
Efficient Delivery of Genome-Editing Proteins In Vitro and In Vivo (2014, background): “We note, however, that the majority of this protein is likely sequestered within endosomes and may not be immediately available to effect genome modification.(4,9)”
Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery. (2019, positive): “22,23 Once inside the cell, LNPs are routed into early endosomes, followed by late endosomes, and ﬁ nally the lysosomes where the mRNA contents are enzymatically degraded.”
The delivery of therapeutic oligonucleotides (2016, background): “(181) found that LNPs were initially taken up by clathrinmediated endocytosis but further accumulation involved macropinocytosis.”