Variability and memory of protein levels in human cells

By Alex Sigal, Ron Milo, A. Cohen, N. Geva-Zatorsky, Yael Klein, Yuvalal Liron, N. Rosenfeld, T. Danon, N. Perzov, Uri Alon. Nature, 2006

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How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Stochastic gene expression and its consequences (2008, methodology): “To examine noise in protein levels in human cells, Sigal et al. (2006) used a clever strategy to fluorescently tag endogenous proteins.”
Systems biology of stem cell fate and cellular reprogramming (2009, background): “However, in many circumstances cell phenotypes are not well defined, and there might be substantial variability between cells, even within a clonal population in a homogeneous environmen”
Complexity and Diversity of the Mammalian Sialome Revealed by Nidovirus Virolectins (2015, background): “…for O-Ac-Sia surface expression or lack thereof, reverted to Sia heterogeneity over time ( 7 days) and a limited number of cell generations (Figure 4D), reminiscent of the short-term phenotypic memory seen in other examples of cell-to-cell variability (Mariani et al., 2010; Sigal et al., 2006).”
Droplet scRNA-seq is not zero-inflated (2019, result): “This was taken as contrasting with fluorescence studies of protein levels in single cells, where no such bimodality was observed (Sigal et al., 2006), and it was concluded that cells had distinct offstates from variable on-states at the RNA level.”
Signalling ballet in space and time (2010, neutral): “In addition, protein concentrations vary between individual cell”