Isolation Procedures for Endophytic Microorganisms

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Colonisation of barley roots by endophytic Fusarium equiseti and Pochonia chlamydosporia: effects on plant growth and disease. (2009, methodology): “Sterilised root pieces were imprinted onto plates with the same medium before plating to evaluate the efficacy of the surface sterilisation method (Hallmann et al., 2006).”
Endophytic fungal diversity: review of traditional and molecular techniques (2012, background): “…(2) surface sterilisation of plant tissue to kill any microorganisms on the host surface, applying different protocols to different tissue types (Hallmann et al. 2006), (3) isolation of endophytic fungi growing out from samples placed on nutrient agar, (4) purification and sporulation of…”