Characterization of OsbZIP23 as a Key Player of the Basic Leucine Zipper Transcription Factor Family for Conferring Abscisic Acid Sensitivity and Salinity and Drought Tolerance in Rice1[W][OA]

By Yong Xiang, Ning Tang, Haomian Du, Haiyan Ye, Lizhong Xiong. Plant Physiology, 2008

658 citations recorded by the source corpus.

OsbZIP23 is a member of the basic leucine zipper (bZIP) transcription factor family in rice (Oryza sativa). Expression of OsbZIP23 is strongly induced by a wide spectrum of stresses, including drought, salt, abscisic acid (ABA), and polyethylene glycol treatments, while other stress-responsive genes of this family are slightly induced only by one or two of the stresses. Transactivation assay in yeast demonstrated that OsbZIP23 functions as a transcriptional activator, and the sequences at the N terminus (amino acids 1–59) and a region close to the C terminus (amino acids 210–240) are required for the transactivation activity. Transient expression of OsbZIP23-green fluorescent protein in onion (Allium cepa) cells revealed a nuclear localization of the protein. Transgenic rice overexpressing OsbZIP23 showed significantly improved tolerance to drought and high-salinity stresses and sensitivity to ABA. On the other hand, a null mutant of this gene showed significantly decreased sensitivity to a high concentration of ABA and decreased tolerance to high-salinity and drought stress, and this phenotype can be complemented by transforming the OsbZIP23 back into the mutant. GeneChip and real-time polymerase chain reaction analyses revealed that hundreds of genes were up- or down-regulated in the rice plants overexpressing OsbZIP23. More than half of these genes have been annotated or evidenced for their diverse functions in stress response or tolerance. In addition, more than 30 genes that are possible OsbZIP23-specific target genes were identified based on the comparison of the expression profiles in the overexpressor and the mutant of OsbZIP23. Collectively, these results indicate that OsbZIP23 functions as a transcriptional regulator that can regulate the expression of a wide spectrum of stress-related genes in response to abiotic stresses through an ABA-dependent regulation pathway. We propose that OsbZIP23 is a major player of the bZIP family in rice for conferring ABA-dependent drought and salinity tolerance and has high potential usefulness in genetic improvement of stress tolerance.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9f1050c831ad443b41661c1bffa119c3122dd4e4

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Citations in the corpus

Genetic engineering and breeding of drought-resistant crops. (2014, background): “Other rice bZIP TFs, such as OsbZIP23 (driven by Ubi1P), OsbZIP46 in the constitutively active form (driven by Ubi1P), and OsbZIP72 (driven by the CaMV35P promoter), have been tested in rice, and overexpression of the genes encoding these TFs resulted in improved DT (64, 107, 123).”
Salinity tolerance in plants: breeding and genetic engineering. (2012, background): “In the same vein, OsbZIP23, a member of basic leucine zipper (bZIP) transcription factor family from rice, when overexpressed results in drought and salt tolerance (Xiang et al., 2008).”
Role of Plant Transcription Factors in Abiotic Stress Tolerance (2011, background): “…– Physiological, Biochemical and Genetic Perspectives 274 Family Gene Transgenic Plants Stress Tolerance References OsbZIP23 Rice Drought, Salinity Xiang et al., 2008 OsAREB1 Rice Drought, Heat Jin et al. 2010 bHLH AtMYC2 Arabidopsis Osmotic stress Abe et al., 2003 MYB AtMYB2 Arabidopsis…”
A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice (2012, background): “As the phytohormone abscisic acid (ABA) plays a crucial role in the adaptive response of plants to abiotic stresses, the pathways leading to the adaptation to stress can be divided into two major categories: ABA-dependent and ABA-independent pathways (Xiang et al., 2008).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “This explains the DR observed in many studies (e.g. Gaxiola et al., 2001; Sakuma et al., 2006; Xiang et al., 2008; Bao et al., 2009).”
General mechanisms of drought response and their application in drought resistance improvement in plants (2014, background): “Several rice bZIP proteins such as OsbZIP23 and the constitutive active form of OsbZIP46 were also characterized to have a high potential in drought resistance improvement in rice [165, 166].”
ABA signaling in stress-response and seed development (2013, background): “Drought tolerance was evaluated according to the yields of transgenic plants overexpressing OsbZIP23 subjected to drought (Xiang et al. 2008).”
Recent Updates on Salinity Stress in Rice: From Physiological to Molecular Responses (2011, background): “Among the four genes encoding bZIPs (OsABF1, OsABI5, OsbZIP23, and OSBZ8; Table 1) associated with salt stress responses, all are induced by salt treatment and also positively responsive to ABA, and only OsABF1 is induced by cold (Hossain et al., 2010; Nakagawa et al., 1996; Xiang et al., 2008; Zou ”