Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition.

By B. Grisart, W. Coppieters, F. Farnir, L. Karim, Christine Ford, P. Berzi, Nadine Cambisano, M. Mni, S. Reid, Patricia Simon and 3 more. Nature reviews genetics, 2002

1,035 citations recorded by the source corpus.

We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition--particularly fat content--to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13 and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.

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Citations in the corpus

• Genomic evaluation of cattle in a multi-breed context ☆ (2014, methodology): “…in accuracy for the Jersey animals were found when using a Bayesian method than when using a GBLUP method, and the largest gain was observed for fat yield, which might be explained by a better ability to estimate the effect of the DGAT1 mutation (Grisart et al., 2002) in the combined dataset.”
• Enzymes of triacylglycerol synthesis and their regulation. (2004, background): “In cows, the polymorphism K232A in DGAT-1 is strongly associated with a quantitative trait locus for decreased milk fat content [169,170].”
• Gene networks driving bovine milk fat synthesis during the lactation cycle (2008, background): “Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P, Spelman R, Georges M, Snell R: Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition.”
• Whole-Genome Regression and Prediction Methods Applied to Plant and Animal Breeding (2013, background): “One of such examples is the DGAT1 gene that has a large effect on fat percentage in dairy cattle (Grisart et al. 2002; Winter et al. 2002).”