Response of alfalfa (Medicago sativa L.) to single and mixed inoculation with phosphate-solubilizing bacteria and Sinorhizobium meliloti

By Lorena B. Guiñazú, J. Andrés, M. F. Del Papa, M. Pistorio, S. Rosas. Biology and Fertility of Soils, 2010

103 citations recorded by the source corpus.

The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain P. putida SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to Bacillus spp. and Pseudomonas spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. Pseudomonas sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with Sinorhizobium meliloti B399 and the Bacillus sp. M7c showed significant increases in the measured parameters. Our results indicating that strains Pseudomonas sp. FM7d and Bacillus sp. M7c can be considered for the formulation of new inoculants.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/9d3cbf6167921928b3f173d0fea57325dc591da0

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Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013) (2014, background): “…Carrillo-Garcia et al. 2000; Dominguez-Nuñez et al. 2012; Egamberdiyeva 2007; Esitken et al. 2003; Fürnkranz et al. 2012; Guetsky et al. 2002a, b; Guiñazú et al. 2010; Hossain and Mårtensson 2008; Jetiyanon et al. 2003; Khan et al. 2003; Kurek and Jaroszuk-Ściseł 2002; Landa et al. 2001; Lopez…”
Bacillus simplex—A Little Known PGPB with Anti-Fungal Activity—Alters Pea Legume Root Architecture and Nodule Morphology When Coinoculated with Rhizobium leguminosarum bv. viciae (2013, background): “concentration via acdS (1-aminocyclopropane-1-carboxylic acid deaminase) activity [22], and/or by acting synergistically with nitrogen-fixing or phosphorous-acquiring symbiotic microbes [11,23–25].”
Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for coinoculation with Rhizobium leguminosarum to improve growth, nodulation, and yield of lentil (2011, background): “The growth and yield promotion of legumes by rhizobial inoculation/coinoculation have been reported by many researchers (Provorov et al. 1998; Pathak et al. 2001; Kannaiyan 2002; Mirza et al. 2007; Remans et al. 2008; Mishra et al. 2009; Guinazu et al. 2010 ).”
Comparison of in vitro solubilization activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth (2010, unknown): “The inoculation of plants with selected PSB to increase native population can mobilize P from poorly available sources and therefore improve plant nutrition (Richardson 2001; Guiñazu et al. 2010).”
Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization (2011, background): “Therefore, P is often regarded as a limiting nutrient in agricultural soils (Guiñazú et al. 2010).”
Manipulating the soil microbiome to increase soil health and plant fertility (2012, background): “plant growth (Cummings 2009; Guiñazú et al. 2009), confer resistance to abiotic stress (Selvakumar et al.”
Rhizosphere interactions: root exudates, microbes, and microbial communities1 (2014, background): “…(Horiuchi et al. 2005), (13) plant-growth-promoting rhizobacteria (PGPR) and rhizobia interaction result in the increase of nodulation efficiency (Guiñazú et al. 2010), and (14) PGPR interaction with mycorrhizae increase colonization efficiency (Hernandez and Chailloux 2004; Vosátka and Gryndler…”