Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy

By Benjamin Schuler, E. Lipman, W. Eaton. Nature, 2002

836 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

The protein folding problem. (2008, background): “Furthermore, single-molecule methods are beginning to explore the conformational heterogeneity of folding (23, 133, 166, 194).”
Protein folding studied by single-molecule FRET. (2008, methodology): “The analysis of the width for the unfolded subpopulation has been a much discussed subject [ 37 ,57] since the first single-molecule folding experiments by Hochstrasser and coworkers [10] and Weiss and coworkers [12], in which the width in excess of that produced by shot noise was attributed to slow”
Photophysics of Fluorescence Probes for Single Molecule Biophysics and Super-Resolution Imaging (2012, neutral): “Interestingly, such FRET histograms are commonly broader than expected from shot-noise considerations even for simple stiff model systems such as double-stranded DNA (86).”
The protein folding 'speed limit'. (2004, background): “[31,32,67,70] have measured the variance in the FRET efficiency distribution in 1 ms snapshots of single freely diffusing unfolded protein molecules (CspTm — a 68-residue cold shock protein).”
Protein folding and misfolding (2003, background): “, some of which can now even be used to follow the behaviour of single molecule”