DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA.

By M. Hofreiter, V. Jaenicke, D. Serre, A. von Haeseler, Svante Pääbo. Nucleic Acids Research, 2001

671 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9bd196079a25b30ff836b67f9b95d2c94f0e95fb

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

In Genetics

In Molecular Biology

Citations in the corpus

Population Demography and Genetic Diversity in the Pleistocene Cave Lion (2015, background): “The identified sequence differences consisted mainly in GC → AT transitions (83%), most likely resulting from PCR nucleotide misincorporations due to post-mortem Cytosine deamination (Hofreiter et al., 2001).”
Genetic analyses from ancient DNA. (2004, background): “For example, when mitochondrial DNA sequences are amplified from late Pleistocene cave bear remains (58), as many as a third of amplification products carry consistent misincorporations.”
This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Mitochondrial genomes reveal an explosive radiation of extinct and extant bears near the Miocene-Pliocene boundary (2008, positive): “Moreover, to ensure sequence accuracy, we determined each sequence position from at least two independent primary PCRs [ 13 ].”
Learning about human population history from ancient and modern genomes (2011, background): “The most substantial such damage appears to be cytosine deamination , in which cytosine is converted into uracil and subsequently 'interpreted' as thymine when sequence”
Neanderthals Revisited: New Approaches and Perspectives (2006, background): “Ancient DNA amplification creates damage that produces mutation artefacts that may artificially enhance differences between Neanderthal and modern human mtDNA (Hansen et al., 2001; Hofreiter et al., 2001; Gutiérrez et al., 2002).”
The top 10 list: criteria of authenticity for DNA from ancient and forensic samples (2003, background): “Most of the DNA extracted and amplified from fossil as well as sub-fossil remains is between 100 and 500 bp in length [4,10].”
Genetic consequences of a demographic bottleneck in the Scandinavian arctic fox (2006, background): “Although misincorporated nucleotides previously have been identified with multiple amplifications in for example DNA molecules from 25 000 /50 000 years old bones and teeth (Hofreiter et al. 2001), there are no such reports in studies on ca 100 years old material (Glenn et al.”