SLAM family markers resolve functionally distinct subpopulations of hematopoietic stem cells and multipotent progenitors
By H. Oguro, Lei Ding, S. Morrison. Cell Stem Cell, 2013
603 citations recorded by the source corpus.
SUMMARY Hematopoietic stem cells (HSCs) and multipotent hematopoietic progenitors (MPPs) are routinely isolated using various markers but remain heterogeneous. Here we show that four SLAM family markers, CD150, CD48, CD229, and CD244, can distinguish HSCs and MPPs from restricted progenitors and subdivide them into a hierarchy of functionally distinct subpopulations with stepwise changes in cell-cycle status, self-renewal, and reconstituting potential. CD229 expression largely distinguished lymphoid-biased HSCs from rarely-dividing myeloid-biased HSCs, enabling prospective enrichment of these HSC subsets. Differences in CD229 and CD244 expression resolved CD150−CD48−/lowLineage−/lowSca-1+c-Kit+ cells into a hierarchy of highly-purified MPPs that retained erythroid and platelet potential but exhibited progressive changes in mitotic activity and reconstituting potential. Use of these markers, and reconstitution assays, showed that conditional deletion of Scf from endothelial cells and perivascular stromal cells eliminated the vast majority of bone marrow HSCs, including nearly all CD229−/low HSCs, demonstrating that quiescent HSCs are maintained by a perivascular niche.
Subfields: Hematology, Immunology
View Paper: https://www.semanticscholar.org/paper/9b4abdb12644c6f1d13631504228d507041c825c
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Citations in the corpus
- Leptin Receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow (2014, neutral): “Conditional deletion of Scf with Lepr-Cre led to the depletion of quiescent HSCs (Ding et al., 2012; Oguro et al., 2013) and conditional deletion of Cxcl12with Lepr-Cre led to HSCmobilization (Ding and Morrison, 2013).”
- Functionally distinct subsets of lineage-biased multipotent progenitors control blood production in normal and regenerative conditions (2015, positive): “Importantly, these populations overlap with other MPP definitions based on reporter gene combinations and different surface markers (Arinobu et al., 2007; Akala et al., 2008; Oguro et al., 2013).”