Braveheart, a long non-coding RNA required for cardiovascular lineage commitment

By Carla A. Klattenhoff, Johanna C. Scheuermann, Lauren E. Surface, Robert K. Bradley, P. Fields, M. Steinhauser, Huiming Ding, Vincent L. Butty, Lillian Torrey, S. Haas and 5 more. Cell, 2013

916 citations recorded by the source corpus.

Summary Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. Using multiple embryonic stem cell (ESC) differentiation strategies, we show that Bvht is required for progression of nascent mesoderm towards a cardiac fate. We find that Bvht is necessary for activation of a core cardiovascular gene network and functions upstream of MesP1 (mesoderm posterior 1), a master regulator of a common multipotent cardiovascular progenitor. We also show that Bvht interacts with SUZ12, a component of Polycomb Repressive Complex 2 (PRC2), during cardiomyocyte differentiation suggesting that Bvht mediates epigenetic regulation of cardiac commitment. Finally, we demonstrate a role for Bvht in maintaining cardiac fate in neonatal cardiomyocytes. Together, our work provides evidence for a long noncoding RNA with critical roles in the establishment of the cardiovascular lineage during mammalian development.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9b2142efd30153b1ae3413c113e11449424fcf1f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

In Molecular Biology

Citations in the corpus

Mechanisms of long noncoding RNA function in development and disease (2016, background): “Braveheart (Bvht) LincRNA IL17b Nucleus Histone modification Cardiac differentiation [45]”
Non-coding RNAs in Development and Disease: Background, Mechanisms, and Therapeutic Approaches. (2016, positive): “1305Physiol Rev • VOL 96 • OCTOBER 2016 • www.prv.org by 10.220.33.5 on N ovem ber 6, 2017 http://physrev.physiology.org/ D ow nloaded from The first example of a lncRNA required for cardiomyocyte lineage commitment is the lncRNA Braveheart (Bvht) (138).”
Circular intronic long noncoding RNAs. (2013, methodology): “Biotin-Labeled RNAs to Pull Down Proteins Biotinylated RNA pull-down assay was performed as described (Klattenhoff et al., 2013; Tsai et al., 2010) with modifications.”
MEG3 long noncoding RNA regulates the TGF-β pathway genes through formation of RNA–DNA triplex structures (2015, neutral): “(b) Bar diagram showing the distribution of T-to-C transitions (indicative of putative RNA–protein contact sites) in input (8,361), EZH2 (18,905) and H3K27me3 (2,651) ChRIP-seq data.”