In Vivo Imaging of Membrane-Associated Glycans in Developing Zebrafish

By Scott T. Laughlin, Jeremy M. Baskin, S. Amacher, Carolyn R. Bertozzi. Science, 2008

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Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation (2014, background): “They can be easily introduced into biomolecules such as glycans (Baskin et al., 2007; Chang et al., 2010; Laughlin et al., 2008), proteins (Beatty et al.”
Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging (2008, background): “Despite these shortcomings, the cycloaddition reaction between azides and ring-strained cyclooctynes has been employed for imaging of cells (4) and zebra fish embryos (10).”
Click hydrogels, microgels and nanogels: emerging platforms for drug delivery and tissue engineering. (2014, background): “In the past years, several cyclooctyne molecules were found to react quickly with azides without a copper catalyst as a result of ring strain and electron-withdrawing fluorine substituents [47,48].”
Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy. (2018, background): “zebrafish, and mice without apparent toxicity or ph ysiological perturbation [55-57].”
Sequential Click Reactions for Synthesizing and Patterning 3D Cell Microenvironments (2009, methodology): “Specifically, azide-labelled cell-surface glycans were reacted with fluorescently labelled cyclooctynes in vivo to enable the visualization of dynamic subcellular development within zebra-fish embryo”