Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging
By N. Devaraj, Ralph Weissleder, S. Hilderbrand. Bioconjugate chemistry, 2008
707 citations recorded by the source corpus.
Bioorthogonal tetrazine cycloadditions have been applied to live cell labeling. Tetrazines react irreversibly with the strained dienophile norbornene forming dihydropyrazine products and dinitrogen. The reaction is high yielding, selective, and fast in aqueous media. Her2/neu receptors on live human breast cancer cells were targeted with a monoclonal antibody modified with a norbornene. Tetrazines conjugated to a near-infrared fluorochrome selectively and rapidly label the pretargeted antibody in the presence of serum. These findings indicate that this chemistry is suitable for in vitro labeling experiments, and suggests that it may prove a useful strategy for in vivo pretargeted imaging under numerous modalities.
Subfields: Molecular Biology, Organic Chemistry, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/af518d2329548baf602a6b31be70c7732f16c320
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
In Organic Chemistry
In Radiology, Nuclear Medicine and Imaging
Citations in the corpus
- Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation (2014, background): “, 2012b), norbornene (Devaraj et al., 2008; Han et al., 2010; Vrabel et al., 2013), cyclobutene (Pipkorn et al.”