Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves

By S. Larrán, A. Perelló, M. R. Simón, V. Moreno. 2002

139 citations recorded by the source corpus.

The present investigation was undertaken in order to select the surface-sterilization technique most efficient for eliminating epiphytes, to document the spectrum of endophytes of healthy leaves from three wheat cultivars in Buenos Aires Province (Argentina) and to determine their infection frequencies at three growth stages. Surface-sterilization with undiluted commercial solution of sodium hypochlorite was reaffirmed as adequate for removing epiphytes on wheat leaves. From the 450 wheat leaf segments incubated, three bacterial isolates and 130 fungal isolates were obtained. From all the isolates, 19 fungal species were identified. Bacterial isolates were characterized as Bacillus sp. There were significant differences between microorganisms, stages of growth, and stages × microorganisms interaction. Differences between cultivars, stages × cultivars, microorganisms × cultivars and for the triple interaction were not significant. Frequency of microorganisms isolated increased with crop age, but it was statistically similar for the three wheat cultivars tested (Klein Centauro, Klein Dragón and Buck Ombú). Rhodotorula rubra, Alternaria alternata, Cladosporium herbarum and Epicoccum nigrum were isolated in the highest frequency. The other microorganisms were present at intermediate or low values. The species isolated may be assigned to three groups: (a) well-known and economically important pathogens of wheat, (b) commonly abundant phylloplane fungi considered to be primary saprobic and minor pathogens and (c) species occasionally present in wheat.

Subfields: Cell Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9a69e0e198971ed9308331227fc4e2840561ac63

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Citations in the corpus

Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots (2005, background): “Although unidentified endophytic yeasts from banana roots (Cao et al. 2002), Acrostichum aureum rhizomes (Maria and Sridhar 2003), rice leaves (Tian et al. 2004), tomato leaves (Rhodotorula sp.) (Larran et al. 2001), wheat leaves (Rhodotorula rubra and Cryptococcus sp.) ( Larran et al. 2002 ) and fr”
Entomopathogenic fungal endophytes (2008, background): “…Roxb., Abutilon indicum (L.) Sweet and Calotropis gigantea (L.) R. Br. (Suryanarayanan et al., 2000), M. acuminata (Cao et al., 2002), wheat (Larran et al., 2002a), oak (Gennaro et al., 2003), Ilex (Takeda et al., 2003), cacti (Suryanarayanan et al., 2005), and in apples (Cammatti-Sartori…”
Potential of yeasts as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters (2006, background): “The isolates obtained belong to the genera Pichia, Rhodotorula, Cryptococcus, and Williopsis (Nakamura et al. 1991; Larran et al. 2002; Nassar et al. 2005).”
Diverse culturable diazotrophic endophytic bacteria from Poaceae plants show cross-colonization and plant growth promotion in wheat (2017, background): “Earlier Bacillus sp., Serratia sp. have been reported from rice and wheat plants (Gyaneshwar et al. 2001; Larran et al. 2002; Mano et al. 2007; Liu et al. 2010), Pseudomonas aeruginosa from pearl millet (Gupta et al. 2013), Brevundimonas sp. from rice (Sun et al. 2008), Sphingomonas sp. from tomato…”
SCREENING OF ANTIMICROBIAL AND ANTIOXIDANT SECONDARY METABOLITES FROM ENDOPHYTIC FUNGI ISOLATED FROM WHEAT (TRITICUM DURUM) (2013, background): “They were isolated from T. aestivum (Larran et al. 2002; Larran et al. 2007), and from rice O. sativa (Tian et al. 2004; Naik et al. 2009).”
Endophytes from wheat as biocontrol agents against tan spot disease (2016, unknown): “S. Larran a,b,⇑, M.R. Simón b, M.V. Moreno c,d, M.P. Santamarina Siurana e, A. Perelló a,d aCentro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, 1900 La Plata, Buenos Aires, Argentina bCerealicultura, Facultad de”