Protein labeling by iTRAQ: A new tool for quantitative mass spectrometry in proteome research

By S. Wiese, K. Reidegeld, H. Meyer, B. Warscheid. Proteomics, 2007

804 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/97a0b529bdbc0fb5682cc5a5ae6c578438cae899

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In Molecular Biology

1. Hans Clevers
2. R. Vries
3. Meritxell Huch
4. L. Valente
5. J. Fernandes
6. I. Johnston
7. Edwin Cuppen
8. B. Aslam

In Spectroscopy

1. B. Aslam
2. M. Khurshid
3. M. Basit
4. M. Rasool
5. M. A. Nisar

Citations in the corpus

• Organoid Models of Human and Mouse Ductal Pancreatic Cancer (2014, methodology): “Protein lysates were processed using amine-reactive isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry (Wiese et al., 2007).”
• What determines growth potential and juvenile quality of farmed fish species (2013, methodology): “Nowadays, more sensitive methodologies are used increasingly, including differential in-gel electrophoresis (DIGE; Link et al. 2006) and iTRAQ labelling and protein separation by LC coupled with MS/MS (Wiese 2007; Martyniuk & Denslow 2009).”
• Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes (2012, methodology): “Protein content confirms cellular origin of exosomes To investigate whether the stress conditions under which our endothelial cells were cultured are reflected in exosome protein content, we performed iTRAQbased quantitative proteomics analysis (37,38).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Two lines of transgenic tobacco plants with a high expression level of AhNRAMP1 were selected for further analysis. hydroponics, after 2–3 weeks of wild-type plants with at least three were grown in hydroponics for ; 1 week and then subjected to Fe deficiency for 9 d. Roots and young leaves were…”
• Proteomics: Technologies and Their Applications. (2017, methodology): “and the substrate benzhydroxamic acid determined by X-ray crystallography; Biochemistry, (1998); 37(22): 8054–8060.”