Magnetic relaxation switches capable of sensing molecular interactions

By J. Perez, Lee Josephson, T. O'Loughlin, D. Högemann, Ralph Weissleder. Nature Biotechnology, 2002

824 citations recorded by the source corpus.

Highly sensitive, efficient, and high-throughput biosensors are required for genomic and proteomic data acquisition in complex biological samples and potentially for in vivo applications. To facilitate these studies, we have developed biocompatible magnetic nanosensors that act as magnetic relaxation switches (MRS) to detect molecular interactions in the reversible self-assembly of disperse magnetic particles into stable nanoassemblies. Using four different types of molecular interactions (DNA–DNA, protein–protein, protein–small molecule, and enzyme reactions) as model systems, we show that the MRS technology can be used to detect these interactions with high efficiency and sensitivity using magnetic relaxation measurements including magnetic resonance imaging (MRI). Furthermore, the magnetic changes are detectable in turbid media and in whole-cell lysates without protein purification. The developed magnetic nanosensors can be used in a variety of biological applications such as in homogenous assays, as reagents in miniaturized microfluidic systems, as affinity ligands for rapid and high-throughput magnetic readouts of arrays, as probes for magnetic force microscopy, and potentially for in vivo imaging.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/96d6d446027bb6f007762566d4dc6f2ce7b46a79

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In Biomedical Engineering

1. Omid Veiseh
2. J. Gunn
3. Fabian Kiessling
4. T. Lammers
5. Miqin Zhang

In Molecular Biology

1. Fabian Kiessling
2. T. Lammers
3. Miqin Zhang

Citations in the corpus

• APPLICATIONS OF MAGNETIC NANOPARTICLES IN BIOMEDICINE (2003, neutral): “system [147, 148].”
• Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging (2009, background): “This flexibility has led to SPION use in magnetic separation [19], biosensor [20,21], in vivo medical imaging [8,22,23], drug delivery [18,24], tissue repair [25], and hyperthermia [26] applications.”
• Magnetic Nanoparticle Sensors (2009, neutral): “proteins CLIO, bench top relaxometer Low nM 300 μL [5,9]”