Protein detection using proximity-dependent DNA ligation assays

By S. Fredriksson, M. Gullberg, Jonas Jarvius, C. Olsson, K. Pietras, S. Gústafsdóttir, A. Ostman, U. Landegren. Nature Biotechnology, 2002

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Direct observation of individual endogenous protein complexes in situ by proximity ligation (2006, methodology): “For this purpose, we adapted the proximity ligation techniqu”
Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall. (2003, methodology): “We therefore analyzed PDGF-B protein levels in conditioned media and cell lysates using a sensitive and specific method (Fredriksson et al. 2002) involving PDGF-B-binding aptamers and proximity-dependent DNA ligation (Fig.”
Homogenous 96-Plex PEA Immunoassay Exhibiting High Sensitivity, Specificity, and Excellent Scalability (2014, methodology): “Upon target binding the probes are brought in proximity, and the two oligonucleotides are extended by a DNA polymerase forming a new sequence that now acts as a unique surrogate marker for the specific antigen [6,7].”
High-throughput proteomics: a methodological mini-review (2022, positive): “Proximity extension assay, as one of proximity-dependent ligation assays, is based on oligonucleotide-linked antibody pairs that have slight af ﬁ nity to each other 64 – 66 .”
Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood (2011, methodology): “The homogenous proximity ligation assay (PLA) has been shown to provide sensitive protein detection in very small sample volumes (1,2).”