Structures and metal-ion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs.

By J. Gifford, M. Walsh, Hans J. Vogel. Biochemical Journal, 2007

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Function-guided protein design by deep manifold sampling (2021, methodology): “When folded using the trRosetta package [30], it forms a helix-loop-helix structural domain at the location of the predicted binding site [31].”
Miro1 Is a Calcium Sensor for Glutamate Receptor-Dependent Localization of Mitochondria at Synapses (2009, neutral): “We have not looked at the effect of Mg2+ on the Miro1-KIF5 interaction, but many EF hands bind some Mg2+ when [Ca2+] is low (Gifford et al., 2007), and changes in [Mg2+] could alter the apparent affinity of Miro1 for Ca2+.”
MICU1 is an Essential Gatekeeper for MCU-Mediated Mitochondrial Ca2+ Uptake That Regulates Cell Survival (2012, positive): “The requirement for two functional EF hands is consistent with MICU1 binding Ca2+ with high affinity (Gifford et al., 2007), as required for it to operate in the low [Ca2+] regime as observed.”
Tertiary alphabet for the observable protein structural universe (2016, background): “Position 18 corresponds to the canonical EF hand loop position 1 (61).”