Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
By Ellen M. Sletten, Carolyn R. Bertozzi. Angewandte Chemie, 2009
2,684 citations recorded by the source corpus.
The study of biomolecules in their native environments is a challenging task because of the vast complexity of cellular systems. Technologies developed in the last few years for the selective modification of biological species in living systems have yielded new insights into cellular processes. Key to these new techniques are bioorthogonal chemical reactions, whose components must react rapidly and selectively with each other under physiological conditions in the presence of the plethora of functionality necessary to sustain life. Herein we describe the bioorthogonal chemical reactions developed to date and how they can be used to study biomolecules.
Subfields: Molecular Biology, Organic Chemistry, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/952b4f041f29b9d8020093e5cfccc44c7a3e53ba
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Citations in the corpus
- Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine (2010, methodology): “With an azide group present, a biotin tag or any other tag can be installed using Huisgen cycloaddition (click) chemistry for a variety of enrichment, detection and sequencing application”
- Cytocompatible Click-based Hydrogels with Dynamically-Tunable Properties Through Orthogonal Photoconjugation and Photocleavage Reactions (2011, background): “Although each click reaction has a number of desirable properties, their true benefit lies in the orthogonality of these reactions with respect to many common reactive groups (for example, amines, alcohols and acids”
- Recombinant Spider Silks—Biopolymers with Potential for Future Applications (2011, positive): “Recent developments concerning the incorporation of non-natural amino acids, native chemical ligation and bio-orthogonal chemistries have expanded the possibilities for site-specific modifications [79,80].”
- Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics (2010, background): “Other conceptually related approaches in the field of “bioorthogonal chemistry” (reviewed recently by Sletten and Bertozzi (68)) may well find applications in connection with cross-linking in the future.”
- Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation (2014, background): “We recommend several excellent published reviews for more comprehensive accounts of the underlying chemistries (Lang and Chin, 2014; Patterson et al., 2014; Prescher and Bertozzi, 2005; Sletten and Bertozzi, 2009).”