Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics
By A. Leitner, T. Walzthoeni, Abdullah Kahraman, Franz Herzog, Oliver Rinner, Martin Beck, R. Aebersold. Molecular & Cellular Proteomics, 2010
468 citations recorded by the source corpus.
Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of cross-linking data for computational modeling. Finally, the impact of protein cross-linking on various biological disciplines is highlighted.
Subfields: Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/1783e2da0f2a0bf97dd57c7b10e27b9d91116d23
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Citations in the corpus
- The beginning of a beautiful friendship: cross-linking/mass spectrometry and modelling of proteins and multi-protein complexes (2010, background): “…al., 2006; Gao et al., 2006; Maiolica et al., 2007; Heymann et al., 2008; Nadeau et al., 2008; Rinner et al., 2008; Singh et al., 2008; Chu et al., 2010; McIlwain et al., 2010; Petrotchenko and Borchers, 2010; Xu et al., 2010) and recently been reviewed (Leitner et al., 2010; Singh et al., 2010).”
- High Sensitivity Crosslink Detection Coupled With Integrative Structure Modeling in the Mass Spec Studio * (2016, methodology): “For development purposes and initial performance testing, we cross-linked bovine serum albumin (BSA) utilizing the protocol in Lietner et al. (15), with minor modifications.”
- OpenStructure: an integrated software framework for computational structural biology (2013, background): “Proteomics cross-links are one such experimental technique (Leitner et al., 2010), in which isotope-labelled cross-linkers such as disuccinimidyl glutarate (DSG) or disuccinimidyl suberate (DSS) are added to the sample.”