Cell type– and brain region–resolved mouse brain proteome

By K. Sharma, S. Schmitt, C. Bergner, Stefka Tyanova, N. Kannaiyan, Natalia Manrique-Hoyos, Karina Kongi, Ludovico Cantuti, U. Hanisch, M. Philips and 3 more. Nature Neuroscience, 2015

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Brain transcriptome and connectome maps are being generated, but an equivalent effort on the proteome is currently lacking. We performed high-resolution mass spectrometry–based proteomics for in-depth analysis of the mouse brain and its major brain regions and cell types. Comparisons of the 12,934 identified proteins in oligodendrocytes, astrocytes, microglia and cortical neurons with deep sequencing data of the transcriptome indicated deep coverage of the proteome. Cell type–specific proteins defined as tenfold more abundant than average expression represented about a tenth of the proteome, with an overrepresentation of cell surface proteins. To demonstrate the utility of our resource, we focused on this class of proteins and identified Lsamp, an adhesion molecule of the IgLON family, as a negative regulator of myelination. Our findings provide a framework for a system-level understanding of cell-type diversity in the CNS and serves as a rich resource for analyses of brain development and function.

Subfields: Immunology, Neurology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/93da7e59e8fb35e600a9a963e3817dccca1ee115

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In Immunology

Marco Prinz

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Citations in the corpus

Revisiting biomarker discovery by plasma proteomics (2017, background): “…the power of cutting edge MS-based proteomics, cell lines can now routinely be quantified to a depth of more than 10,000 different proteins in a relatively short time, sometimes even without any fractionation (Mann et al, 2013; Richards et al, 2015; Sharma et al, 2015; Bekker-Jensen et al, 2017).”
Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation (2019, background): “Only recently, comprehensive profiling of bulk populations of CNS macrophages, including of entire transcriptomes (10) and proteomes (11), has helped to uncover several myeloid cell states during homeostasis (12) and disease (13).”