Critical Evaluation of Two Primers Commonly Used for Amplification of Bacterial 16S rRNA Genes

By Jeremy A. Frank, C. Reich, Shobha Sharma, J. S. Weisbaum, B. Wilson, G. Olsen. Applied and Environmental Microbiology, 2008

1,668 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/929bbb1de26ddb50a0b7e96bc6f4a53345d61f4c

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Citations in the corpus

Microbial community profiling for human microbiome projects: Tools, techniques, and challenges. (2009, background): “For example, although some widely used primers such as 8F (Meyer et al. 2004; Edwards et al. 2006; Frank et al. 2008), 337F (Huse et al.”
Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies (2011, background): “V13 sequences were nearly undetectable in the raw sequence pool. This result can be explained by a one base mismatch in the middle of the forward primer sequence, which is a common variant among many bacteria and can be recovered using a degenerate form of the primer”
Bacterial Communities in Women with Bacterial Vaginosis: High Resolution Phylogenetic Analyses Reveal Relationships of Microbiota to Clinical Criteria (2012, background): “vaginalis 16S rRNA gene sequence, a key bacterium in the vaginal niche [67], potentially leading to lower representation of these sequences in some studies [17,61,62].”
High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease (2011, neutral): “often underrepresented Actinobacteria phylum [65,66].”
Microbial metagenomics in aquaculture: a potential tool for a deeper insight into the activity (2017, methodology): “Variant regions V1 to V9 of the 16S and V1 to V9 of the 18S rRNA genes (rDNAs) have been used for years for taxonomic identification (Lane et al. 1981; Frank et al. 2008).”