Effects of the presence or absence of a protein corona on silica nanoparticle uptake and impact on cells.

By A. Leśniak, Federico Fenaroli, M. Monopoli, Christoffer Åberg, Kenneth A. Dawson, Anna Salvati. ACS Nano, 2012

1,004 citations recorded by the source corpus.

Nanoparticles enter cells through active processes, thanks to their capability of interacting with the cellular machinery. The protein layer (corona) that forms on their surface once nanoparticles are in contact with biological fluids, such as the cell serum, mediates the interactions with cells in situ. As a consequence of this, here we show that the same nanomaterial can lead to very different biological outcomes, when exposed to cells in the presence or absence of a preformed corona. In particular, silica nanoparticles exposed to cells in the absence of serum have a stronger adhesion to the cell membrane and higher internalization efficiency, in comparison to what is observed in medium containing serum, when a preformed corona is present on their surface. The different exposure conditions not only affect the uptake levels but also result in differences in the intracellular nanoparticle location and impact on cells. Interestingly, we also show that after only one hour of exposure, a corona of very different nature forms on the nanoparticles exposed to cells in the absence of serum. Evidence suggests that these different outcomes can all be connected to the different adhesion and surface properties in the two conditions.

Subfields: Biomaterials, Biomedical Engineering, Surfaces, Coatings and Films

View Paper: https://www.semanticscholar.org/paper/90a177e9ff8473c00a208effa05669c37f677cf4

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In Biomaterials

1. W. Chan
2. C. Walkey
3. Andrew Emili
4. Jonathan B. Olsen
5. Hongbo Guo
6. S. Bhattacharjee
7. C. Murphy

In Biomedical Engineering

1. W. Chan
2. S. Bhattacharjee

In Surfaces, Coatings and Films

1. W. Chan
2. C. Walkey

Citations in the corpus

• In vitro approaches to assess the hazard of nanomaterials (2017, background): “heat-inactivation) and the source can strongly effect NMs interactions with cells (Lesniak et al., 2010; Lesniak et al., 2012).”
• DLS and zeta potential - What they are and what they are not? (2016, background): “A common example to this phenomenon is the adsorption of the proteins on the surface of the NPs creating the characteristic protein corona [42,43].”
• Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle (2013, background): “Once taken up, NPs can accumulate in the lysosomes [46,47], intracellular vacuoles as reported in the case of SWCNT uptake by HeLa cells [48], or cytoplasm of cells as observed for copolymer NP [49].”
• Protein corona fingerprinting predicts the cellular interaction of gold and silver nanoparticles. (2014, background): “Surface-adsorbed serum proteins may either promote cell association or inhibit it.(26,58) The sign of the bi coefficients from the model (eq 2) reflects the correlation between each adsorbed serum protein and cell association.”
• A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives (2017, neutral): “Particle size [87], particle shape [97], particle surface properties [98], and biological fluid properties and composition affect the corona composition and thus the adverse effects on human health and the environment [135,136].”