Evaluation of Copper Oxide Nanoparticles Toxicity Using Chlorophyll a Fluorescence Imaging in Lemna gibba
By F. Perreault, A. Oukarroum, L. Pirastru, Louka Sirois, W. G. Matias, R. Popovic. 2010
88 citations recorded by the source corpus.
Copper oxide nanoparticles (CuO NPs), used in antifouling paints of boats, are released in the environment and can induce toxicity to aquatic organisms. In this report, we used chlorophyll a fluorescence imaging to evaluate CuO NPs toxicity in Lemna gibba. This approach allowed to evaluate the differential effect of CuO NPs on photosynthesis of whole L. gibba plants. Exposure to 0.1 to 0.4 g/L CuO NPs during 48h induced strong inhibition of photosynthetic processes resulting in a decrease of plant growth. By using fluorescence imaging, different photosynthetic parameters were evaluated simultaneously in microplate conditions. Imaging of <path id="x46" d="M493 503l-29 -4q-13 56 -28 80q-13 22 -35.5 29.5t-78.5 7.5h-76q-22 0 -27.5 -5t-5.5 -27v-238h103q66 0 82.5 12t25.5 62h29v-193h-29q-9 54 -25.5 67t-82.5 13h-103v-181q0 -64 15 -78.5t84 -19.5v-28h-272v28q61 5 74.5 19.5t13.5 78.5v399q0 64 -13 78t-72 19v28 h440q1 -40 10 -147z" /> fluorescence yield showed the decrease of leaf photosynthetic active surface for whole plants exposed to CuO NPs. This method showed that CuO NPs inhibited photosystem II maximal, photosystem II operational quantum yields, and photochemical quenching of fluorescence associated with electron transport. Nonphotochemical fluorescence quenching as an indicator of energy dissipation not used in photosynthesis was shown to be increased by the effect of CuO NPs. Such approach in microplate conditions provides synchronous high repetition measurements for numerous plants. This study may give a reliable methodological approach to evaluate toxicity risk of NPs in aquatic ecosystems.
Subfields: Materials Chemistry, Ocean Engineering, Pollution
View Paper: https://www.semanticscholar.org/paper/908ec1698999ba36a5faa3019b9c892d8d5fe463
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In Materials Chemistry
Citations in the corpus
- Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa (2016, background): “Similarly, Perreault et al. (2010) reported that Lemna gibba, on exposure to CuO NPs, showed a decrease in qP with increase in the nonphotochemical quenching.”
- CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat (2012, background): “2010) and CuO NPs (400 mg/l) in Lemna gibba plants (Perreault et al. 2010).”
- Chemistry, Biochemistry of Nanoparticles, and Their Role in Antioxidant Defense System in Plants (2015, unknown): “4 g/L nCuO has been investigated (Perreault et al. 2010).”
- Biological Synthesis of Copper Nanoparticles and its impact-a Review (2014, background): “4 g/L CuO Nps for 48h, induced strong inhibition of photosynthetic processes resulting in a decrease of plant growth in Lemna gibba has been reported [97].”