nCoV-2019 sequencing protocol v1

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Temporal Detection and Phylogenetic Assessment of SARS-CoV-2 in Municipal Wastewater (2020, methodology): “To verify that the qRT-PCR results reflect bona fide detection of SARS-CoV-2 rather than priming from an unintended template, we repeated the PCR using 10 primer pairs that tile across the SARS-CoV-2 genome.(22) These primers were designed to target conserved regions of the genome that flank polymor”