Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

By Conrad L. Schocha, Keith A. Seifertb, Sabine Huhndorfc, Vincent Robertd, John L. Spougea, C. A. Levesqueb, W. Chenb, Fungal Barcoding Consortiuma. Proceedings of the National Academy of Sciences of the United States of America, 2012

5,125 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/8d3f88df3add3b382f8ac046d1bc3b97144e5e81

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Citations in the corpus

Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity (2015, background): “…COI for animals (Hebert et al., 2003), the plastid ribulose 1,5- bisphosphate carboxylase gene (rbcL) and the maturase K gene (matK) for plants (Hollingsworth et al., 2009) and the internal transcribed spacer (ITS) for fungi (Nilsson et al., 2009; Bellemain et al., 2010; Schoch et al., 2012).”
Species in lichen-forming fungi: balancing between conceptual and practical considerations, and between phenotype and phylogenomics (2021, methodology): “While the ITS has been chosen as the standard barcoding marker for fungi (Schoch et al. 2012), employed in almost all studies including presumed species pairs (Table 3), it may present limited resolution when lineages diverged recently (Lücking et al.”
High-level classification of the Fungi and a tool for evolutionary ecological analyses (2018, background): “Species-level molecular identification of fungi takes advantage of the Internal Transcribed Spacer (ITS) region of ribosomal RNA (rRNA) gene (Gardes and Bruns 1996; Kõljalg et al. 2005; Schoch et al. 2012; Nilsson et al. 2014).”
How will the ‘molecular revolution’ contribute to biological recording? (2015, background): “…cytochrome c oxidase subunit I gene (COI) for animals (Hebert, Ratnasingham, & deWaard, 2003), the chloroplast ribulose biphosphate carboxylase gene (rbcL) gene for plants (Hollingsworth & Andra Clark, 2009), and the ribosomal internal transcribed spacer (ITS) for fungi (Schoch et al., 2012).”
Global biogeography of marine fungi is shaped by the environment (2016, background): “The 18S in general (Schoch et al., 2012), and the V9 region in particular (Hartmann et al., 2010), are considerably more conserved than the ITS cistron.”
Species of the Colletotrichum gloeosporioides and C. boninense complexes associated with olive anthracnose (2014, background): “…marker because they can be easily amplified and sequenced and, compared to alternative genes, they have the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation (Schoch et al., 2012).”
A method for simultaneous measurement of soil bacterial abundances and community composition via 16S rRNA gene sequencing (2016, methodology): “357 Further testing is needed to determine the usefulness of our approach for other applications, 358 such as when analyzing fungal communities via sequencing the internal transcribed spacer 359 region (Schoch et al. 2012) or when analyzing microbial communities found in other 360 environments (incl”
Evolution of nutritional modes of Ceratobasidiaceae (Cantharellales, Basidiomycota) as revealed from publicly available ITS sequences (2013, background): “Barcoding with the Internal Transcribed Spacer (ITS) region of the ribosomal DNA locus has become a standard means of identification in fungi (Schoch et al. 2012).”
Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi (2014, background): “Citation details: Schoch,C.L., Robbertse,B., Robert,V. et al. Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi.”
Phylogeny, identification and nomenclature of the genus Aspergillus (2014, background): “8S-ITS2) is the official DNA barcode for fungi (Schoch et al. 2012), because it is the most frequently sequenced marker in fungi and has primers that work universally.”
One stop shop: backbones trees for important phytopathogenic genera: I (2014) (2014, background): “These studies showed that the universal fungal DNA barcoding marker–the ITS region (Schoch et al. 2012)–is sufficient for Choanephora species identification (Table 6, Fig.”