Unbiased stereological estimation of the number of neurons in the human hippocampus

By M. West, H. Gundersen. 1990

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Citations in the corpus

Human Hippocampal Neurogenesis Persists throughout Aging (2018, neutral): “…of the hippocampus, treated tissue at 80°C for 1 hr and at low pH to obtain deparaffinization and antigen retrieval, and assessed cell density without using stereology, which is the gold standard, given that cell density does not necessarily reflect total cell number (West and Gundersen, 1990).”
Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum (2017, methodology): “After the recovery period, slices were either transferred to the recording chamber or stored in the oxygenated sucrose ACSF for up to 5 h at room temperature.”
Prefrontal cortex in humans and apes: a comparative study of area 10. (2001, methodology): “The total number of neurons was determined for area 10 by applying the optical fractionator, a modern stereological method for obtaining estimates of total numbers of neurons in a cortical area (West and Gundersen, 1990; West et al., 1991; Hyman et al., 1998).”
Variation in hippocampal neuron number with age and brain volume. (1998, methodology): “Table 3 Neuron number (×10 6 ) for each of the subregions of the hippocampus The effect of cerebral volume has not been considered in past analyses of age-related changes in CA1 neuron number (West and Gundersen, 1990; Simic et al., 1997).”
Design-based stereology in neuroscience. (2005, methodology): “Estimated total numbers of neurons within a BROI can be obtained by the " V Ref ϫN V " method (West and Gundersen , 1990), i.e. by calculating the product of the mean neuron density within the unbiased virtual counting spaces (N V ) and the global volume of the BROI (V Ref ; estimated with…”
The Search for True Numbers of Neurons and Glial Cells in the Human Brain: A Review of 150 Years of Cell Counting (2016, methodology): “Thus, utilization of the Giemsa stain predates the adoption of this stain by Gundersen, West, and Pakkenberg for their resin sections in the 1980s and 1990s (e.g., Gundersen et al., 1988; West and Gundersen, 1990; Regeur et al., 1994a).”
Sequence of information processing for emotions based on the anatomic dialogue between prefrontal cortex and amygdala (2007, methodology): “We then employed standard stereological procedures to estimate the areal and laminar density of boutons, using the optical fractionator according to the method described by West and Gundersen (e.g., Gundersen et al., 1988; West and Gundersen, 1990).”
Volume and number of neurons of the human hippocampal formation in normal aging and Alzheimer's disease (1997, methodology): “According to the nomogram and suggestions given in West and Gundersen (1990), we used point-counting grids with different interpoint distances for different subdivisions: 2 mm for subiculum and CA1, 1 mm for CA3-2 and hilus, and 0.2 mm for the granule cell layer.”
Presenilin 1 mutants impair the self-renewal and differentiation of adult murine SVZ-neuronal progenitors via cell autonomous mechanisms involving Notch signaling (2010, methodology): “The volume of the ventricle was determined according to Cavalieri’s principle (West and Gundersen, 1990), by multiplying the sum of the cross sectional areas by the spacing T between sampled sections.”