Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.

By T. Tiller, E. Meffre, S. Yurasov, M. Tsuiji, Michel C. Nussenzweig, Hedda Wardemann. JIM - Journal of Immunological Methods, 2008

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Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients’ B Cells (2020, neutral): “On the other hand, single-cell RT-PCR combined with fluorescence-activated cell sorting (FACS) or optofluidics platform, such as Beacon (Berkley Light), could obtain antibody sequences in several days by performing nested PCR on single antigen-binding memory B cells after single-cell sorting (Tiller”
Characterization of neutralizing antibodies from a SARS-CoV-2 infected individual (2020, methodology): “The VH and VL sequences were recovered using gene specific primers and cycling conditions previously described(Tiller et al., 2008).”
A Combination of Human Broadly Neutralizing Antibodies against Hepatitis B Virus HBsAg with Distinct Epitopes Suppresses Escape Mutations (2020, methodology): “B cell purification, labeling, and sorting were as previously described (Escolano et al., 2019; Robbiani et al., 2017; Tiller et al., 2008; von Boehmer et al., 2016).”
Flow Cytometry Reveals that H5N1 Vaccination Elicits Cross-Reactive Stem-Directed Antibodies from Multiple Ig Heavy-Chain Lineages (2014, methodology): “Using established methods (18), we amplified the immunoglobulin genes by RT-PCR and sequenced the resulting DNA fragments, obtaining sequences for 77 heavy-chain genes and the corresponding light chain for 66 of these (Fig.”