Cell permeabilization using a non-thermal plasma

By M. Leduc, David Guay, Richard L. Leask, S. Coulombe. 2009

223 citations recorded by the source corpus.

The atmospheric pressure glow discharge torch (APGD-t) is a miniature pulsed RF (13.56 MHz) plasma source specifically designed to efficiently produce and transport reactive species to and interact with biological samples. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. We observe that this maximum radius is below 6.5 nm. We also show that no degradation occurs when a plasmid DNA suspended in culture media is plasma-treated at the operating conditions leading to cell permeabilization. The plasma-assisted DNA transfection of the HeLa cells with the hrGFP-II-1 plasmid using the APGD-t is demonstrated. Local transfection efficiencies obtained are as high as 35%.

Subfields: Electrical and Electronic Engineering, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/8a51eb4abc2b4936486a5bd2f7bff43ce55bb972

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Citations in the corpus

• Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development (2018, background): “In contrast, the media protected the DNA sample to a large extent (Leduc et al. 2009).”
• Quantitative detection of plasma-generated radicals in liquids by electron paramagnetic resonance spectroscopy (2013, unknown): “[17–21].”
• Plasma medicine: an introductory review (2009, unknown): “• Cell permeabilization using a non-thermal plasma [23].”