A high performance liquid chromatographic assay of Mefloquine in saliva after a single oral dose in healthy adult Africans
By G. Gbotosho, C. Happi, Omowunmi Lawal, A. Sijuade, A. Sowunmi, A. Oduola. Malaria Journal, 2012
413 citations recorded by the source corpus.
Mefloquine-artesunate is a formulation of artemisinin based combination therapy (ACT) recommended by the World Health Organization and historically the first ACT used clinically. The use of ACT demands constant monitoring of therapeutic efficacies and drug levels, in order to ensure that optimum drug exposure is achieved and detect reduced susceptibility to these drugs. Quantification of anti-malarial drugs in biological fluids other than blood would provide a more readily applicable method of therapeutic drug monitoring in developing endemic countries. Efforts in this study were devoted to the development of a simple, field applicable, non-invasive method for assay of mefloquine in saliva. A high performance liquid chromatographic method with UV detection at 220 nm for assaying mefloquine in saliva was developed and validated by comparing mefloquine concentrations in saliva and plasma samples from four healthy volunteers who received single oral dose of mefloquine. Verapamil was used as internal standard. Chromatographic separation was achieved using a Hypersil ODS column. Extraction recoveries of mefloquine in plasma or saliva were 76-86% or 83-93% respectively. Limit of quantification of mefloquine was 20 ng/ml. Agreement between salivary and plasma mefloquine concentrations was satisfactory (r = 0.88, p < 0.001). Saliva:plasma concentrations ratio was 0.42. Disposition of mefloquine in saliva paralleled that in plasma, making salivary quantification of mefloquine potentially useful in therapeutic drug monitoring.
Subfields: Analytical Chemistry, Pharmacology, Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/8a24e29dff7e95b783a2d574f512e74eae7ecfdc
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Public Health, Environmental and Occupational Health
Citations in the corpus
- Qualitative Methods in Implementation Research: An Introduction (2019, methodology): “…2013), Theoretical Domains Framework (Atkins et al., 2017; Lawton et al., 2016), as well as with the Consolidated Framework for Implementation Research (CFIR; Damschroder et al., 2017; Damschroder & Lowery 2013; Keith et al., 2017), which offers CFIR-guided tools for data collection and analysis.”
- Making sense of implementation theories, models and frameworks (2015, neutral): “[134] and Theoretical Domains Framework [135].”
- A systematic review of the use of the Consolidated Framework for Implementation Research (2015, background): “Damschroder LJ, Lowery JC. Evaluation of a large-scale weight management program using the consolidated framework for implementation research (CFIR).”
- Comparison of rapid vs in-depth qualitative analytic methods from a process evaluation of academic detailing in the Veterans Health Administration (2019, methodology): “Damschroder LJ, Aron DC, Keith RE, Kirsh SR, Alexander JA, Lowery JC. Fostering implementation of health services research findings into practice: a consolidated framework for advancing implementation science.”
- The updated Consolidated Framework for Implementation Research based on user feedback (2022, neutral): “qualitative data for each construct [86, 87].”
- An introduction to implementation science for the non-specialist (2015, methodology): “Damschroder and Lowery conducted an evaluation of a weight management program in USVA, funded by the QUERI program described above [43, 44].”