Engineered CRISPR-Cas9 nucleases with altered PAM specificity

By ジョン，ジェー．キース, クレインスティヴァー，ベンジャミン. 2016

122 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/89a9d487fc7a6193bff1a1e745af2b90e8aab795

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

1. Kira S. Makarova
2. Rodolphe Barrangou
3. Eugene V. Koonin
4. Bernd Zetsche
5. Feng Zhang
6. T. Yamano
7. Ryan T. Leenay
8. I. Slaymaker

Citations in the corpus

• Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, methodology): “PAM (Figure 5B) that mirrors its lower enrichment score (Figure 5A) and negligible repression for the non-canonical PAMs (Kleinstiver et al., 2015).”
• Crystal structure of Cpf1 in complex with guide RNA and target DNA (2016, background): “Structure-guided engineering and direct evolution approaches have led to variants of Cas9 with enhanced target specificity (Slaymaker et al., 2016; Kleinstiver et al., 2016) or altered PAM requirements (Kleinstiver et al., 2015a, 2015b).”