The human mitochondrial transcriptome
By Tim R. Mercer, Shane J. Neph, Marcel E. Dinger, J. Crawford, Martin A. Smith, A. Shearwood, E. Haugen, C. Bracken, O. Rackham, John A. Stamatoyannopoulos and 2 more. Cell, 2011
798 citations recorded by the source corpus.
Summary The human mitochondrial genome comprises a distinct genetic system transcribed as precursor polycistronic transcripts that are subsequently cleaved to generate individual mRNAs, tRNAs and rRNAs. Here we provide a comprehensive analysis of the human mitochondrial transcriptome across multiple cell lines and tissues. Using directional deep sequencing and parallel analysis of RNA ends, we demonstrate wide variation in mitochondrial transcript abundance and precisely resolve transcript processing and maturation events. We identify previously undescribed transcripts, including small RNAs, and observe the enrichment of several nuclear RNAs in mitochondria. Using high-throughput in vivo DNaseI footprinting, we establish the global profile of DNA-binding protein occupancy across the mitochondrial genome at single nucleotide resolution, revealing regulatory features at mitochondrial transcription initiation sites and functional insights into disease-associated variants. This integrated analysis of the mitochondrial transcriptome reveals unexpected complexity in the regulation, expression, and processing of mitochondrial RNA, and provides a resource for future studies of mitochondrial function (accessed at mitochondria.matticklab.com).
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/884f6dd427c3a6955656b7fa267c8ed3a684b803
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In Molecular Biology
Citations in the corpus
- Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq (2021, neutral): “The co-regulation of mitochondrial genes tended to reflect function, with the exception of the bicistronic mRNAs ND4L/ND4 and ATP8/ATP6 (Mercer et al., 2011).”
- The Mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance (2015, positive): “See also Figure S6 for total activity and Figure S7 for similar data in C57BL/6 mice. of sORFs in the mtDNA (Mercer et al., 2011).”