Measuring stress in wildlife: techniques for quantifying glucocorticoids

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On the use of non‐invasive hormone research in uncontrolled, natural environments: the problem with sex, diet, metabolic rate and the individual (2012, background): “…Möstl, Rettenbacher & Palme 2005; Palme 2005; Touma & Palme 2005; Wielebnowski & Watters 2007; Scott et al. 2008; Sheriff, Krebs & Boonstra 2010; Sheriff et al. 2011), but unfortunately, papers without these essential validations continue to get published even in high-quality journals (e.g.…”
Stress Indicators in Fish (2016, background): “…hormones are typically measured by either radioimmunoassay or enzyme-linked immunoassay in plasma or tissue homogenates (Pottinger, 2008; Sheriff et al., 2011) Glucocorticoids can also be measured in urine and feces, and so can be extracted and measured in water samples (Ellis et al.,…”
The ecology of stress: effects of the social environment (2013, background): “…effects of reproductive condition, seasonality or recent extreme weather events on HPA axis activity or responsiveness (Kenagy & Place 2000; Romero 2002; Romero et al. 2008) or to the effects of trapping/handling stress on glucocorticoid levels (Kenagy & Place 2000; Sheriff et al. 2011a).”