Extraction and purification of microbial DNA from soil and sediment samples

By C. Roose-Amsaleg, É. Garnier-Sillam, M. Harry. 2001

258 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/873286e5f8ebfcc8cf79d4af2cb45e00b5fc7e11

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In Ecology

In Molecular Biology

Citations in the corpus

Molecular approaches to study the insect gut symbiotic microbiota at the ‘omics’ age (2010, methodology): “Two strategies have been popular for metagenomic DNA isolation, and they are the cell recovery method and the direct lysis method (Roose-Amsaleg et al., 2001).”
Volatile organic compounds (VOCs) in soils (2010, background): “Ruiz et al. (1998) investigated the adsorption of n-hexane, n-heptane, n-octane, toluene, xylene, ethylbenzene, methyl ethyl ketone, and of water vapor on sand, clay, and limestone.”
Extraction of DNA from soil (2003, background): “Both approaches have advantages and disadvantages related to DNA yields, DNA purity for molecular purposes, and the unbiased representation of the entire microbial diversity [10,58].”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, background): “Extraction requirements and recommendations DNeasy PowerSoil® kits are widely used and have been shown to be well optimised for DNA extraction from a variety of soils, including compost, sediment, clay and acidic soils (Roose-Amsaleg et al. 2001; Tedersoo et al. 2014).”
Culture-independent molecular techniques for soil microbial ecology (2010, methodology): “Once cells have been lysed, a number of methods may be used to extract and purify the nucleic acids, several using commercial kits (Roose-Amsaleg et al., 2001; Robe et al., 2003; Thakuria et al., 2009).”
Functional and molecular responses of soil microbial communities under differing soil management practices (2004, background): “The two extraction–purification protocols, targeting bacterial community DNA obtained after centrifuge separation of bacteria from soil particle, or total DNA, that is extracellular soil DNA and plant debris DNA, besides DNA from living microbiota, may affect yields, composition, extractability, and”