Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

By P. Walsh, D. A. Metzger, Mohammed Taha. BioTechniques, 2013

6,248 citations recorded by the source corpus.

Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most types of samples. The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and phenol-chloroform extraction. DNA extracted from bloodstains seems less prone to contain PCR inhibitors when prepared by this method. The Chelex method has been used with amplification and typing at the HLA DQ alpha locus to obtain the DQ alpha genotypes of many different types of samples, including whole blood, bloodstains, seminal stains, buccal swabs, hair and post-coital samples. The results of a concordance study are presented in which the DQ alpha genotypes of 84 samples prepared using Chelex or using conventional phenol-chloroform extraction are compared. The genotypes obtained using the two different extraction methods were identical for all samples tested.

Subfields: Genetics

View Paper: https://www.semanticscholar.org/paper/859cc0ca9ae244b4141d737c02d6b346dc4191b6

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In Genetics

Citations in the corpus

Sperm number and velocity affect sperm competition success in the guppy (Poecilia reticulata) (2011, methodology): “Genomic DNA was extracted using a Chelex protocol (Walsh et al. 1991) for adults and newborns.”
A molecular phylogeny of the genus Apis suggests that the Giant Honey Bee of the Philippines, A. breviligula Maa, and the Plains Honey Bee of southern India, A. indica Fabricius, are valid species (2010, methodology): “DNA was obtained by grinding the hind leg of individual bees in 5% Chelex solution (1 mm Tris HCl pH 7.6, 0.1 mm EDTA pH 8) and incubation at 100◦C for 15 min (Walsh et al., 1991).”
Highly restricted gene flow and deep evolutionary lineages in the giant clam Tridacna maxima (2009, methodology): “Total genomic DNA was isolated using the Chelex method, following the protocol from Walsh et al. (1991) .”
Molecular systematics of Indochinese primates (2007, neutral): “DNA from the samples was extracted using methods as outlined in Sambrook et al. (1989), Walsh et al. (1991) , or by applying the Qiagen DNA Mini Kit or Qiagen DNA Stool Mini Kit.”
No effect of parental quality or extrapair paternity on brood sex ratio in the blue tit (Parus caeruleus) (2001, methodology): “Sexing techniques DNA was made available for amplification either by a standard proteinase-K/phenol chloroform extraction (Kawasaki, 1990) or through a Chelex resin-based technique (Walsh et al., 1991).”
Sequence divergence of mitochondrial DNA indicates cryptic biodiversity in Octopus vulgaris and supports the taxonomic distinctiveness of Octopus mimus (Cephalopoda: Octopodidae) (2000, methodology): “A modification of the Chelex protocol ( Walsh et al. 1991 ) for DNA extraction is described, resulting in a dissolution of even formalin-fixed tissue with a minimum of hands-on time.”
Circumpolar dispersal by rafting in two subantarctic kelp-dwelling crustaceans (2010, methodology): “We extracted total genomic DNA from leg or pleotelson muscle tissue of the invertebrates using a standard Chelex method (Walsh et al. 1991).”
Estimating population size by genotyping remotely plucked hair: the Eurasian badger (2004, methodology): “Hair samples were extracted using a Chelex protocol (Chelex®-100, Bio-Rad, Hercules, CA; Walsh, Metzger & Higuchi 1991 ).”
Application of an inexpensive and high‐throughput genomic DNA extraction method for the molecular ecology of zooplanktonic diapausing eggs (2008, methodology): “A chelating resin method (using Chelex® 100 resin or InstaGene matrix, Bio-Rad) (Walsh et al. 1991), originally developed to extract DNA from forensic material, has been, and remains to this date, the most widely used method to prepare DNA from diapausing stages and individual zooplankton in rotifer”