Application of an inexpensive and high‐throughput genomic DNA extraction method for the molecular ecology of zooplanktonic diapausing eggs

By J. Montero-Pau, A. Gómez, J. Muñoz. 2008

314 citations recorded by the source corpus.

We describe the application of a simple, low‐cost, and effective method of DNA extraction (hot sodium hydroxide and Tris, HotSHOT) to the diapausing propagules of continental aquatic invertebrates for its use in PCR amplification. We illustrate the use of the technique in cladocerans, rotifers, anostracans, notostracans, and copepod diapausing eggs. We compare the performance of the HotSHOT technique to the currently most widely used method for DNA extraction of zooplankton eggs and individuals, the chelating resin (or Chelex) technique. The HotSHOT technique overcomes several of the problems posed by Chelex and permits easy optimization for its use with 96‐well plates for high‐throughput DNA extraction and subsequent genetic characterization. We foresee a wide use of this technique in the future from DNA barcoding of diapausing stages to the genetic characterization of the diapausing egg banks of continental aquatic invertebrates.

Subfields: Ecology, Nature and Landscape Conservation

View Paper: https://www.semanticscholar.org/paper/747b5a0f004b5e916cc4992ea7e41deaba5a31f6

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. M. Cristescu
2. H. MacIsaac
3. S. Bailey
4. Elizabeta Briski
5. Francesca Gherardi
6. A. Ricciardi
7. P. Hulme

In Nature and Landscape Conservation

1. S. Bailey
2. M. Cristescu
3. H. MacIsaac
4. A. Ricciardi
5. P. Hulme
6. Petr Pyšek

Citations in the corpus

• Use of DNA barcoding to detect invertebrate invasive species from diapausing eggs (2011, methodology): “Previous testing of the HotSHOT method (Montero-Pau et al. 2008) for DNA extraction success on freshly cultured Daphnia pulex eggs from our lab resulted in 96% success (E.”