Large-scale analysis of the yeast proteome by multidimensional protein identification technology
By M. Washburn, Dirk Wolters, John R. Yates III. Nature Biotechnology, 2001
4,480 citations recorded by the source corpus.
Subfields: Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/85675f464599530e0cf6612991f6bb498e4e8574
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- Alexey I. Nesvizhskii
- R. Aebersold
- John R. Yates III
- Steven P. Gygi
- Joshua E. Elias
- R. Nusse
- Matthias Mann
- Hans Clevers
In Spectroscopy
- Alexey I. Nesvizhskii
- John R. Yates III
- R. Aebersold
- Steven P. Gygi
- Joshua E. Elias
- Matthias Mann
- C. Ruse
- A. Nakorchevsky
Citations in the corpus
- Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation (2007, background): “, shotgun proteomics is perhaps closest to reaching this goa”
- Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis. (2006, methodology): “We have taken a systems biology approach aided by the sensitivity of MudPIT proteomics to identify transient interactions that contribute to CFTR folding, trafficking, and regulatory pathways—the CFTR interactome.”
- A method for the comprehensive proteomic analysis of membrane proteins (2003, methodology): “Shotgun methods provide useful alternatives to gels, whereby proteins are first digested into more complex peptide mixtures that are then analyzed directly by liquid chromatography coupled to mass spectrometry (LC-MS”
- Toward a Human Blood Serum Proteome (2002, methodology): “Filters for SEQUEST Results—SEQUEST results were filtered (Table I) with criteria similar to those developed by Yates and co-workers (31, 36).”
- Microglia promote learning-dependent synapse formation through BDNF (2013, methodology): “We first performed a quantitative proteomic screen from whole-brain protein extracts by shotgun multidimensional liquid chromatography–tandemmass spectrometry (LCLC-MS/MS) high-resolution mass spectrometric proteome analysis (Washburn et al., 2001).”
- AN ACCURATE MASS TAG STRATEGY FOR QUANTITATIVE AND HIGH THROUGHPUT PROTEOME MEASUREMENTS (2002, methodology): “Very recently this approach was used to identify 1484 yeast proteins via an automated 2D capillary liquid chromatography (LC)-tandem MS strategy([10]).”