Nanometric three-dimensional tracking of individual quantum dots in cells

By L. Holtzer, T. Meckel, T. Schmidt. 2007

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SINGLE-PARTICLE TRACKING (2009, background): “A weak cylindrical lens can be inserted in the optical path to encode z; the image is circular in the focal plane but elliptical above and below the focal plane, with the long axis shifting as one goes from above to below [101,102].”
Fast, single-molecule localization that achieves theoretically minimum uncertainty (2010, methodology): “Extension to 3D Astigmatic Imaging Following Holtzer [7] and using σ0 as usual the PSF near focus can be described by a 2D Gaussian with a z-dependent standard deviation σ(z) = σ0 √ 1 + z2 d2 +A z3 d2 +B z4 d2 , (15) where σ0 is the in focus standard deviation, d is the depth of focus (a constant fo”
Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function (2009, background): “Improvements in 3D localization beyond this limit are also possible by using astigmatism (10, 11), defocusing (12), or simultaneous multiplane viewing (13).”
Mass production and dynamic imaging of fluorescent nanodiamonds. (2008, background): “The excellent photostability and high brightness of the material also make long-term, three-dimensional trackin”