Molecular tools for clone identification: the case of the grapevine cultivar ‘Traminer’

By S. Imazio, Massimo Labra, F. Grassi, M. Winfield, Mauro Bardini, A. Scienza. 2002

109 citations recorded by the source corpus.

In viticulture, biotype identification problems have traditionally been solved using ampelography, ampelometry and chemical traits analysis. However, these tools have resulted in several false attributions, in particular when used at the clonal level. The availability of relatively cheap, reliable and reproducible tools to identify genetic differences at the clonal level would greatly facilitate the work of clonal patenting. In this work, 24 accessions of ‘Traminer’ cultivars were characterized using molecular markers. Three different approaches were applied: simple sequence repeats (SSR), amplified fragment length polymorphism (AFLP) and methyl-sensitive amplified length polymorphism (MSAP). Results showed that SSRs were not a powerful tool for clonal distinction. In contrast, the AFLP technique was able to distinguish 16 out of the 24 cultivars, even though the average similarity was high (97.1%). The MSAP technique was used to evaluate qualitative differences in the degree of DNA methylation among clones. Results suggest that morphological differences among clones are probably due to the synergetic effect of genetic and epigenetic modifications, and that clonal identification could be greatly improved using molecular tools such as AFLP and MSAP.

View Paper: https://www.semanticscholar.org/paper/83c0fb5e5845948a978021ca8927186e8d34ade4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

1. Séverine Bory
2. M. Duval
3. P. Besse
4. M. Grisoni

Citations in the corpus

• Biodiversity and preservation of vanilla: present state of knowledge (2008, background): “These are responsible for the regulation of gene expression, and were shown to be transmitted through vegetative propagation (Xiong et al. 1999; Bretó et al. 2001; Imazio et al. 2002; Kalisz and Purugganan 2004).”