Foundations of Confocal Scanned Imaging in Light Microscopy

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A guided tour into subcellular colocalization analysis in light microscopy (2006, background): “The first light disc is called the Airy disc (Inoué, 1995).”
DeconvolutionLab2: An open-source software for deconvolution microscopy. (2017, background): “…470 nm) and for the standard numerical aperture NA ¼ 1 : 4 and diffraction index n i ¼ 1 : 51 nm, the Rayleigh criterion predicts that it is impossible to observe details smaller than about 0 : 61 k NA (cid:2) 200 nm in the lateral sections and 2 n i k NA 2 (cid:2) 700nm along the optical axis [1].”
Accuracy and precision in quantitative fluorescence microscopy (2009, methodology): “Subtracting a constant background value from intensity measurements does not change the variance due to Poisson noise; the presence of background therefore reduces image SNR.”