A guided tour into subcellular colocalization analysis in light microscopy

By S. Bolte, Fabrice Cordelières. Journal of Microscopy, 2006

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Accuracy and precision in quantitative fluorescence microscopy (2009, background): “Bleed-through of one fluorophore’s emission through the filter set of another fluorophore can occur when a specimen is labeled with multiple fluorophores whose excitation and emission spectra overlap (Fig. 5; Ploem, 1999; Bolte and Cordelieres, 2006; Rietdorf and Stelzer, 2006).”
Quantifying colocalization by correlation: The Pearson correlation coefficient is superior to the Mander's overlap coefficient (2010, background): “5 (10) and the MOC’s threshold is apparently 0.”