Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants

By D. Traver, B. Paw, K. Poss, W. Penberthy, Shuo Lin, Leonard I. Zon. Nature Immunology, 2003

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Transparent adult zebrafish as a tool for in vivo transplantation analysis. (2008, background): “The Transparent casper Mutant Reveals Timing and Homing of Hematopoietic Stem Cells to the Marrow Compartment Prior work from our laboratory has demonstrated that the transplantable hematopoietic stem/progenitor (HSPC) marrow population resides within the adult kidney, and can be isolated using flow”
Reverse genetic screening reveals poor correlation between Morpholino-induced and mutant phenotypes in zebrafish (2014, background): “The Tg(fli1a:egfp), Tg(kdrl:egfp), Tg(gata1a:dsRed), Tg(flt4:mCitrine), and Tg(flt1:tdTomato) transgenic lines have been described elsewhere (Beis et al., 2005; Bussmann et al., 2010; Lawson and Weinstein, 2002; Traver et al., 2003; van Impel et al., 2014).”
A dynamic epicardial injury response supports progenitor cell activity during zebrafish heart regeneration. (2006, neutral): “stein, 2002), flk1:EGFP (Jin et al., 2005), or b-actin 2:EGFP ( Traver et al., 2003 ) transgenic strains or crosses between the strains to gen-”
Hematopoietic stem cells derive directly from aortic endothelium during development (2010, positive): “These data are consistent with our previous demonstration that the β actin promoter is highly expressed in leukocytes, but is silenced upon erythropoietic differentiation 20 .”