SNP Genotyping Using the Sequenom MassARRAY iPLEX Platform

By Stacey Gabriel, L. Ziaugra, D. Tabbaa. Current Protocols in Human Genetics, 2009

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High-Throughput SNP Genotyping to Accelerate Crop Improvement (2014, methodology): “On the other hand, other SNP systems use alternate techniques, such as the Sequenom MassARRAY® iPLEX system that employs MALDI-TOF mass spectrometry to identify SNP allele across up to 36 multiplexed products (Gabriel et al. 2009).”
Current trends in microsatellite genotyping (2011, background): “…relied on very large (>20) multiplexes (e.g. Hill et al. 2009; Chen et al. 2010), whereas simultaneous PCR amplification of 35–40 PCR products is routinely achieved in the case of SNPs (e.g. Gabriel et al. 2009; Buggs et al. 2010), demonstrating that problems of primer competition can be overcome.”