Preparation of nucleosome core particle from recombinant histones.

By K. Luger, T. Rechsteiner, T. Richmond. 1999

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Citations in the corpus

• Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation. (2002, methodology): “Recombinant Xenopus octamer and oligonucleosomes were prepared as described (Luger et al. 1999), where 5S ribosomal DNA repeats were used for assembly of oligonucleosomes.”
• Alteration of the nucleosomal DNA path in the crystal structure of a human nucleosome core particle (2005, methodology): “The histone octamers were reconstituted into NCPs using the 146 bp palindromic DNA fragment (24,25).”
• Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP. (2009, unknown): “His-H2A:H2B dimers were reconstituted and purified as described (Luger et al., 1999).”
• Epigenetic regulation of neurogenesis in the adult mammalian brain (2011, background): “The building blocks of chromatin, nucleosome core particles, each consists of approximately 147 bp of DNAwrapped around two copies of four distinct histone proteins: H2A, H2B, H3 and H4 (Luger et al., 1999).”
• γ-H2AX Dephosphorylation by Protein Phosphatase 2A Facilitates DNA Double-Strand Break Repair (2005, methodology): “Mononucleosomes containing human g-H2AX were made following the method of ( Luger et al., 1999 ) with details in the Supplemental Data.”
• Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution. (2002, methodology): “laevis histones and 146 and 147 bp palindromic DNA fragments derived from human a-satellite DNA, as described previously.(1,43) The 146 bp and 147 bp”
• Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein. (2002, methodology): “Octamers and oligonucleosomes with recombinant Xenopus histones were prepared as described by Luger et al. (1999). Plasmid containing 5S ribosomal DNA repeats was used for assembly of oligonucleosomes.”
• Histone Tails and the H3 αN Helix Regulate Nucleosome Mobility and Stability (2007, unknown): “pressed and purified as previously described (33).”