DNA Fingerprinting of Tetraploid Cherry Germplasm Using Simple Sequence Repeats

By C. Cantini, A. Iezzoni, W. Lamboy, M. Boritzki, D. Struss. 2001

160 citations recorded by the source corpus.

ADDITIONAL INDEX WORDS. microsatellites, Prunus cerasus, P. fruticosa ABSTRACT. The U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS) tetraploid cherry ( Prunus L. sp.) collection at Geneva, N.Y., contains ≈75 accessions of sour cherry (P. cerasus L.), ground cherry (P. fruticosa Pall.), and their hybrids. Accurate and unambiguous identification of these accessions is essential for germplasm preservation and use. Simple sequence repeats (SSRs) are currently the markers of choice for germplasm fingerprinting because they characteristically display high levels of polymorphism. Recently SSR primer pairs from sweet cherry ( P. avium L.), sour cherry, and peach ((P. persica L. Batsch (Peach Group)) have been reported. Ten SSR primer pairs were tested on 59 tetraploid cherry accessions to determine if they could differentiate among the accessions. Scorable SSR fragments were produced with all primer-accession combinations. The cherry accessions exhibited high levels of polymorphism with 4 to 16 different putative alleles amplified per primer pair. Most of the putative alleles were rare with frequencies <0.05. Heterozygosity values ranged from 0.679 to 1.00, while gene diversity values ranged from 0.655 to 0.906. The primer pairs differentiated all but two of the 59 cherry accessions. Based upon the ability of the SSR data to differentiate the cherry accessions and the high level of gene diversity, we propose that all the tetraploid cherry accessions in the USDA/ARS collection be fingerprinted to provide a mechanism to verify the identity of the individual accessions. The fingerprinting data are available on the World Wide Web (http://www.ars-grin.gov/gen/cherry.html) so that other curators and scientists working with cherry can verify identities and novel types in their collections and contribute to a global database.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/7cf4b01ae919b2fa7a853a641b0d88765bd62722

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In Molecular Biology

1. A. Wünsch
2. D. Potter
3. S. Southwick
4. R. Ahmad
5. N. Bassil
6. R. Botta
7. S. Mehlenbacher
8. E. Dirlewanger

In Plant Science

1. A. Wünsch
2. S. Southwick
3. T. Gradziel
4. R. Ahmad
5. E. Dirlewanger
6. D. Struss
7. M. Boritzki
8. J. Hormaza

Citations in the corpus

• Development of a second-generation genetic linkage map for peach [Prunus persica (L.) Batsch] and characterization of morphological traits affecting flower and fruit (2006, methodology): “…and AFLP analysis Several sources of Prunus SSRs were tested for their polymorphism in the F2 progeny (Cipriani et al. 1999; Sosinski et al. 2000; Cantini et al. 2001; Aranzana et al. 2002; Dirlewanger et al. 2002; Lopes et al. 2002; Yamamoto et al. 2002; Clarke and Tobutt 2003; Decroocq et al.…”
• Cultivar identification and genetic fingerprinting of temperate fruit tree species using DNA markers (2002, methodology): “Thus, Downey and Iezzoni (2000) used one primer pair developed in sweet cherry, and 2 developed in sour cherry and peach to fingerprint 66 black cherry (Prunus serotina) genotypes; Cantini et al. (2001) used 10 primer pairs from peach, sweet and sour cherry to identify 59 tetraploid sour cherry (Pru”
• Microsatellite markers in hazelnut : Isolation, characterization, and cross-species amplification (2005, result): “…pairs is high and is comparable to that obtained from other out-crossed woody perennials, such as the 9.5 alleles per locus reported for Persea (Lavi et al., 1994), 14.3 for Quercus L. (Dow et al., 1995), 10.7 for Prunus cerasus L. (Cantini et al., 2001), and 12.1 for Malus (Hokanson et al., 1998).”
• An extended interspecific gene pool available to peach and almond breeding as characterized using simple sequence repeat (SSR) markers (2003, background): “Recently, SSR primer pairs generated from different Prunus species have been reported (Cipriani et al. 1999; Cantini et al. 2001; Downey and Iezzoni 2000; Hormaza, 2002; Serrano et al. 2002; Sosinski et al. 2000; Testolin et al., 2000).”
• Analysis of Sweet Cherry (Prunus avium L.) Cultivars Using SSR and AFLP Markers (2003, background): “Microsatellites have been recognized as a good source of genetic markers in cherry (Cantini et al., 2001; Downey and Iezzoni, 2000), but a major limitation of SSRs is the time and cost 9394-MolB 904 9/29/03, 10:19:38 AM 905J. AMER.”
• Sequencing and annotation of the evergrowing locus in peach [Prunus persica (L.) Batsch] reveals a cluster of six MADS-box transcription factors as candidate genes for regulation of terminal bud formation (2008, methodology): “Gene content of the EVG locus Gene content of the EVG locus was predicted by combined analysis of the results of GenScan, GeneMark.hmm, UDP96-0050 PMS6722 BPPCT01632 Pchgms2837 EPDCU287240 CPPCT2943 EVG/evg50 Pchgms4152 Pchgms12 Pchgms2953 BPPCT02856 UDA3160 G1 Fig.”