Microsatellite markers in hazelnut : Isolation, characterization, and cross-species amplification

By N. Bassil, R. Botta, S. Mehlenbacher. 2005

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Three microsatellite-enriched libraries of the european hazelnut (Corylus avellana L.) were constructed: library A for CA repeats, library B for GA repeats, and library C for GAA repeats. Twenty-fi ve primer pairs amplifi ed easy-to-score single loci and were used to investigate polymorphism among 20 C. avellana genotypes and to evaluate cross-species amplifi cation in seven Corylus L. species. Microsatellite alleles were estimated by fl uorescent capillary electrophoresis fragment sizing. The number of alleles per locus ranged from 2 to 12 (average = 7.16) in C. avellana and from 5 to 22 overall (average = 13.32). With the exception of CAC-B110, di-nucleotide SSRs were characterized by a relatively large number of alleles per locus (≥5), high average observed and expected heterozygosity (Ho and He > 0.6), and a high mean polymorphic information content (PIC ≥ 0.6) in C. avellana. In contrast, tri-nucleotide microsatellites were more homozygous (Ho = 0.4 on average) and less informative than di-nucleotide simple sequence repeats (SSRs) as indicated by a lower mean number of alleles per locus (4.5), He (0.59), and PIC (0.54). Cross-species amplifi cation in Corylus was demonstrated. These microsatellite markers were highly heterozygous and polymorphic and differentiated among genotypes of C. avellana irrespective of geographical origin. They will aid in fi ngerprinting genotypes of the european hazelnut and other Corylus species, genome mapping, and genetic diversity assessments.

Subfields: Cell Biology, Endocrinology, Nutrition and Dietetics

View Paper: https://www.semanticscholar.org/paper/977eaf2d5ed4c7c6fbfcbedc6984ea3b67bfd6b1