State-of-the-art gene-based therapies: the road ahead

By M. Kay. Nature reviews genetics, 2011

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Current advances and future perspectives in extrusion-based bioprinting. (2016, background): “To date, both in vivo and ex vivo gene therapy have been used in tissue repair [213]; however, bioprinting genes have been studied to a limited extent [67].”
Non viral vectors in gene therapy- an overview. (2015, background): “technical challenges and limitations to successful non-Viral gene transfer The major technical limitations or critical steps in attaining a successful gene therapy are categorized into [5-8]: efficiency of vector transport and unloading into target cells, perseverance, activity, immune response, reg”