Plant Genome Size Measurement with DNA Image Cytometry

By B. Vilhar, Johann Greilhuber, J. Koce, E. Temsch, M. Dermastia. 2001

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Genome size in Bulgarian Centaurea s.l. (Asteraceae) (2006, methodology): “The nuclear DNA content was determined from Feulgen-stained root tip meristems using video-based DNA image densitometry on the CIRES (Cell Image Retrieval and Evaluation System, version 3.1, of KONTRON ELEKTRONIK, Munich, Germany) (compare Dimitrova et al. 1999, Vilhar et al. 2001).”
Plant DNA flow cytometry and estimation of nuclear genome size. (2005, background): “DNA image cytometry may be seen as an electronic alternative to microspectrophotometry, where the absorption is determined by estimating grey level values of pixels of an image grabbed by a video camera (Vilhar et al., 2001).”
Self-incompatibility in the Grasses (2000, background): “Positional cloning will be substantially more difficult in rye and H. bulbosum, where the estimates of genome size are considerably larger than for Phalaris or Lolium, at around 15 pg ( Vilhar et al. 2001; Jakob et al. 2004).”