Analyzing proteome topology and function by automated multidimensional fluorescence microscopy

By W. Schubert, B. Bonnekoh, A. Pommer, L. Philipsen, R. Böckelmann, Y. Malykh, H. Gollnick, Manuela Friedenberger, M. Bode, A. Dress. Nature Biotechnology, 2006

481 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/7b0829f29e632622c2553d9cb25d9bf1301d06a6

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Citations in the corpus

Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging (2017, unknown): “Approaches have been developed to overcome such limitations (Schubert et al., 2006; Gerdes et al., 2013), but these protocols have required multiple stain/ strip/wash cycles of the antibodies that can be time consuming or lead to sample degradation over the iterations.”
Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. (2007, unknown): “Recently, a method using multiple rounds of immunolabeling, fluorescent tag bleaching, and restaining of 5 mm cryostat sections, has underlined the importance and power of multiplexing approaches (Schubert et al., 2006).”
Histopathological Image Analysis: A Review (2009, methodology): “Another recently developed sequential imaging method known as MELC [30] has the ability to produce images of the same specimen with up to 100 proteins by utilizing the photo-bleaching characteristics of the fluorescent dyes.”
Coordinated Cellular Neighborhoods Orchestrate Antitumoral Immunity at the Colorectal Cancer Invasive Front (2019, methodology): “Multiplexed imaging technologies allow capturing many parameters of single cells while preserving their spatial location (Agasti et al., 2017; Angelo et al., 2014; Gerdes et al., 2013; Giesen et al., 2014; Gut et al., 2018; Huang et al., 2013; Lin et al., 2018; Moffitt et al., 2018; Saka et al., 201”
Visualization of image data from cells to organisms (2010, unknown): “Color coding becomes useless when tens or hundreds of channels must be visualized simultaneously (for example, in multi-epitope-ligand cartography 18 ).”
Automated subcellular location determination and high-throughput microscopy. (2007, background): “A powerful new robotic technology for sequential fluorescent staining of as many as a hundred proteins in fixed tissue samples has been shown to enable discrimination of disease-specific localization patterns (Schubert et al., 2006).”